PAB2 Antibody

Datasheet

Code	CSB-PA017365XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes:PAB2 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 35.5 kDa Observed band size: 35 kDa
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana PAB2 Polyclonal antibody

Uniprot No.

P42731

Target Names

PAB2

Alternative Names

Polyadenylate-binding protein 2 (PABP-2) (Poly(A)-binding protein 2) PAB2 At4g34110 F28A23.130

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PAB2 protein (36-292aa)

Immunogen Species

Arabidopsis thaliana

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the cytoplasm, affects both translation and mRNA decay. Stimulates translation by interaction with translation initiation factor eIF4G, a subunit of the cap-binding complex eIF4F, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. During infection with potyvirus TuMV, acts as a potential integral component of the viral replicase complex that could play an important role in the regulation of potyviral RNA-dependent RNA polymerase (RdRp).

Gene References into Functions

Poly(A) Binding Protein binds to uridylated oligo(A) tails and determines the length of U-extensions added by URT1. PMID: 26972004
PAB2 was partially retargeted to the nucleolus in the presence of TuMV VPg-Pro. In addition, the membrane association of PAB2 during TuMV infection resulted from the internalization of the host protein in 6K-VPg-Pro-induced vesicles. PMID: 17670821

Subcellular Location

Cytoplasm. Nucleus. Note=In TuMV-infected plants, partially retargeted in cytoplasmic virus-induced vesicles and in the nucleolus.

Protein Families

Polyadenylate-binding protein type-1 family

Tissue Specificity

Expressed in all organs (at the protein level) but under distinct spatial and temporal regulation within each organ.

Database Links

KEGG: ath:AT4G34110

STRING: 3702.AT4G34110.1

UniGene: At.25252

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PAB2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background