Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAN2 Polyclonal antibody

Uniprot No.

P53010

Target Names

PAN2

Alternative Names

PAN2 antibody; YGL094C antibody; PAN2-PAN3 deadenylation complex catalytic subunit PAN2 antibody; EC 3.1.13.4 antibody; PAB1P-dependent poly(A)-specific ribonuclease antibody; Poly(A)-nuclease deadenylation complex subunit 2 antibody; PAN deadenylation complex subunit 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAN2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping by DCP1-DCP2 and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails, trimming the tails from their synthesized length to the slightly shorter, apparently messenger-specific length found on newly exported mRNAs. PAN cooperates with protein kinase DUN1 in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress.

Gene References into Functions

PAN2 and PAN3 are not required for mRNA poly(A) tail length control during de novo synthesis but may act in conjuction with Pab1p at a later stage in mRNA metabolism. PMID: 15894541
The exonuclease subunit Pan2 does not bind RNA by itself, but binds to an asymmetric Pan3 homodimer via an extensive, high-affinity interaction. PMID: 24872509
Accumulation of polyadenylated mRNAs at sites of transcription occurs in strains lacking the PAN2 or PAN3 subunits of the poly(A) nuclease PAN. PAN is identified as an important factor that couples 3' processing of pre-mRNAs to export. PMID: 15630021

Subcellular Location

Cytoplasm.

Protein Families

Peptidase C19 family, PAN2 subfamily

Database Links

KEGG: sce:YGL094C

STRING: 4932.YGL094C

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PAN2 Antibody

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