PCL2 Antibody

Code CSB-PA326567XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PCL2 Polyclonal antibody
Uniprot No.
Target Names
PCL2
Alternative Names
PCL2 antibody; CLN4 antibody; YDL127W antibody; D2223 antibody; PHO85 cyclin-2 antibody; Cyclin HCS26 homolog antibody; G1/S-specific cyclin PCL2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PCL2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
G1/S-specific cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Essential for the control of the cell cycle at the G1/S (start) transition. Together with cyclin PCL1, positively controls degradation of sphingoid long chain base kinase LCB4. The PCL2-PHO85 cyclin-CDK holoenzyme phosphorylates LCB4, which is required for its ubiquitination and degradation. PCL2-PHO85 also phosphorylates RVS167, linking cyclin-CDK activity with organization of the actin cytoskeleton.
Gene References into Functions
  1. Phosphorylation of Bni4 by Pcl-Pho85 is necessary for its localization to the bud neck, and the bud neck structure can be disrupted by overexpressing BNI4 in pcl1Deltapcl2Delta mutant cells. PMID: 19458192
Subcellular Location
Cytoplasm. Nucleus. Note=Localizes to sites of polarized growth, namely the incipient bud site, the bud tip and the bud neck.
Protein Families
Cyclin family, PCL1,2 subfamily
Database Links

KEGG: sce:YDL127W

STRING: 4932.YDL127W

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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