Recombinant Saccharomyces cerevisiae PHO85 cyclin-2 (PCL2)

Code CSB-YP326567SVG
MSDS
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Source Yeast
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Code CSB-EP326567SVG
MSDS
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Source E.coli
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Code CSB-EP326567SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326567SVG
MSDS
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Source Baculovirus
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Code CSB-MP326567SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PCL2
Uniprot No.
Alternative Names
PCL2; CLN4; YDL127W; D2223; PHO85 cyclin-2; Cyclin HCS26 homolog; G1/S-specific cyclin PCL2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-308
Target Protein Sequence
MSNYEALLKF NRKAVSKEMV QYLASTTASI IKIKKTNSMI DIALPAPPLT KFINRLIKHS NVQTPTLMAT SVYLAKLRSI IPSNVYGIET TRHRIFLGCL ILAAKTLNDS SPLNKHWAEY TDGLLILREV NTIERELLEY FDWDVTISTD DLITCLSPFL KPIKEEQLYK SQRDCRTLKN FSAQEKDIVN KTSISHSRSS SNMSIPSLAS TSTLSTLESR RSNLSNYSNR IRTLPELHES NNISDKFSPR TYNIDSKHDN KENRPIPTIK PFNFSKARPV ILKTGLNKQI IKEDTKVKKS NWSNYFKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
G1/S-specific cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Essential for the control of the cell cycle at the G1/S (start) transition. Together with cyclin PCL1, positively controls degradation of sphingoid long chain base kinase LCB4. The PCL2-PHO85 cyclin-CDK holoenzyme phosphorylates LCB4, which is required for its ubiquitination and degradation. PCL2-PHO85 also phosphorylates RVS167, linking cyclin-CDK activity with organization of the actin cytoskeleton.
Gene References into Functions
  1. Phosphorylation of Bni4 by Pcl-Pho85 is necessary for its localization to the bud neck, and the bud neck structure can be disrupted by overexpressing BNI4 in pcl1Deltapcl2Delta mutant cells. PMID: 19458192
Subcellular Location
Cytoplasm. Nucleus. Note=Localizes to sites of polarized growth, namely the incipient bud site, the bud tip and the bud neck.
Protein Families
Cyclin family, PCL1,2 subfamily
Database Links

KEGG: sce:YDL127W

STRING: 4932.YDL127W

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