PDI1 Antibody

Code CSB-PA017342XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PDI1 Polyclonal antibody
Uniprot No.
Target Names
PDI1
Alternative Names
PDI1 antibody; MFP1 antibody; TRG1 antibody; YCL043C antibody; YCL313 antibody; YCL43C antibody; Protein disulfide-isomerase antibody; PDI antibody; EC 5.3.4.1 antibody; Thioredoxin-related glycoprotein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PDI1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein disulfide isomerase of ER lumen required for formation of disulfide bonds in secretory and cell-surface proteins and which unscrambles non-native disulfide bonds. Forms a complex with MNL1 to process unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response.
Gene References into Functions
  1. The results suggest that PDI flexibility enables it to interact with many different partner molecules of widely different sizes and shapes. PMID: 27616289
  2. Data show that binding of mannosidase Htm1 to protein disulfide isomerase Pdi1 is required for endoplasmic reticulum (ER)-associated protein degradation (ERAD). PMID: 27053108
  3. ERO1 is enzymatically active even with its regulatory disulfides intact, and further activation of ERO1 by reduction of the regulatory disulfides requires the reduction of non-catalytic Cys(90)-Cys(97)disulfide in PDI1. PMID: 26846856
  4. Coexpression of Kar2p, Pdi1p and Ero1p gave a synergistic effect on CPY expression PMID: 24146283
  5. Pdi1p is the key regulator of Ero1p activity. PMID: 22412017
  6. analysis of the oxidative activity of yeast Ero1p on protein disulfide isomerase and related oxidoreductases of the endoplasmic reticulum PMID: 20348090
  7. The introduction of PDI improves secretory proteins in Pichia pastoris secretion 4-7 times, which may act synergistically to increase recombinant protein yields. PMID: 16889384

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Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Protein disulfide isomerase family
Database Links

KEGG: sce:YCL043C

STRING: 4932.YCL043C

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