Recombinant Saccharomyces cerevisiae Protein disulfide-isomerase (PDI1)

Code CSB-YP017342SVG
MSDS
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Source Yeast
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Code CSB-EP017342SVG
MSDS
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Source E.coli
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Code CSB-EP017342SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017342SVG
MSDS
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Source Baculovirus
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Code CSB-MP017342SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PDI1
Uniprot No.
Alternative Names
PDI1; MFP1; TRG1; YCL043C; YCL313; YCL43C; Protein disulfide-isomerase; PDI; EC 5.3.4.1; Thioredoxin-related glycoprotein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
29-522
Target Protein Sequence
PE DSAVVKLATD SFNEYIQSHD LVLAEFFAPW CGHCKNMAPE YVKAAETLVE KNITLAQIDC TENQDLCMEH NIPGFPSLKI FKNSDVNNSI DYEGPRTAEA IVQFMIKQSQ PAVAVVADLP AYLANETFVT PVIVQSGKID ADFNATFYSM ANKHFNDYDF VSAENADDDF KLSIYLPSAM DEPVVYNGKK ADIADADVFE KWLQVEALPY FGEIDGSVFA QYVESGLPLG YLFYNDEEEL EEYKPLFTEL AKKNRGLMNF VSIDARKFGR HAGNLNMKEQ FPLFAIHDMT EDLKYGLPQL SEEAFDELSD KIVLESKAIE SLVKDFLKGD ASPIVKSQEI FENQDSSVFQ LVGKNHDEIV NDPKKDVLVL YYAPWCGHCK RLAPTYQELA DTYANATSDV LIAKLDHTEN DVRGVVIEGY PTIVLYPGGK KSESVVYQGS RSLDSLFDFI KENGHFDVDG KALYEEAQEK AAEEADADAE LADEEDAIHD EL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein disulfide isomerase of ER lumen required for formation of disulfide bonds in secretory and cell-surface proteins and which unscrambles non-native disulfide bonds. Forms a complex with MNL1 to process unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response.
Gene References into Functions
  1. The results suggest that PDI flexibility enables it to interact with many different partner molecules of widely different sizes and shapes. PMID: 27616289
  2. Data show that binding of mannosidase Htm1 to protein disulfide isomerase Pdi1 is required for endoplasmic reticulum (ER)-associated protein degradation (ERAD). PMID: 27053108
  3. ERO1 is enzymatically active even with its regulatory disulfides intact, and further activation of ERO1 by reduction of the regulatory disulfides requires the reduction of non-catalytic Cys(90)-Cys(97)disulfide in PDI1. PMID: 26846856
  4. Coexpression of Kar2p, Pdi1p and Ero1p gave a synergistic effect on CPY expression PMID: 24146283
  5. Pdi1p is the key regulator of Ero1p activity. PMID: 22412017
  6. analysis of the oxidative activity of yeast Ero1p on protein disulfide isomerase and related oxidoreductases of the endoplasmic reticulum PMID: 20348090
  7. The introduction of PDI improves secretory proteins in Pichia pastoris secretion 4-7 times, which may act synergistically to increase recombinant protein yields. PMID: 16889384

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Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Protein disulfide isomerase family
Database Links

KEGG: sce:YCL043C

STRING: 4932.YCL043C

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