Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PEP5 Polyclonal antibody

Uniprot No.

P12868

Target Names

PEP5

Alternative Names

PEP5 antibody; END1 antibody; VAM1 antibody; VPL9 antibody; VPS11 antibody; VPT11 antibody; YMR231W antibody; YM9959.13 antibody; E3 ubiquitin-protein ligase PEP5 antibody; EC 2.3.2.27 antibody; Carboxypeptidase Y-deficient protein 5 antibody; Histone E3 ligase PEP5 antibody; RING-type E3 ubiquitin transferase PEP5 antibody; Vacuolar biogenesis protein END1 antibody; Vacuolar morphogenesis protein 1 antibody; Vacuolar protein sorting-associated protein 11 antibody; Vacuolar protein-targeting protein 11 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PEP5 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21. Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation.

Gene References into Functions

The results highlight the importance of posttranslational histone regulatory mechanisms that employ multiple E3 ubiquitin ligases such as Pep5 to ensure excess histone degradation and thus contribute to the maintenance of genomic stability. PMID: 22570702
The C-terminal domain of Vps11 is a key integrator of CORVET (class C core vacuole/endosome tethering complex) assembly, Rab signaling, and endosomal and lysosomal traffic. PMID: 21325627

Subcellular Location

Vacuole membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

VPS11 family

Database Links

KEGG: sce:YMR231W

STRING: 4932.YMR231W

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PEP5 Antibody

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