Recombinant Saccharomyces cerevisiae Vacuolar membrane protein PEP5 (PEP5), partial

Code CSB-YP321027SVG
MSDS
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Source Yeast
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Code CSB-EP321027SVG
MSDS
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Source E.coli
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Code CSB-EP321027SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321027SVG
MSDS
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Source Baculovirus
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Code CSB-MP321027SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEP5
Uniprot No.
Alternative Names
PEP5; END1; VAM1; VPL9; VPS11; VPT11; YMR231W; YM9959.13; E3 ubiquitin-protein ligase PEP5; EC 2.3.2.27; Carboxypeptidase Y-deficient protein 5; Histone E3 ligase PEP5; RING-type E3 ubiquitin transferase PEP5; Vacuolar biogenesis protein END1; Vacuolar morphogenesis protein 1; Vacuolar protein sorting-associated protein 11; Vacuolar protein-targeting protein 11
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21. Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation.
Gene References into Functions
  1. The results highlight the importance of posttranslational histone regulatory mechanisms that employ multiple E3 ubiquitin ligases such as Pep5 to ensure excess histone degradation and thus contribute to the maintenance of genomic stability. PMID: 22570702
  2. The C-terminal domain of Vps11 is a key integrator of CORVET (class C core vacuole/endosome tethering complex) assembly, Rab signaling, and endosomal and lysosomal traffic. PMID: 21325627
Subcellular Location
Vacuole membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
VPS11 family
Database Links

KEGG: sce:YMR231W

STRING: 4932.YMR231W

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