PHO1 Antibody

Code CSB-PA844594XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PHO1 Polyclonal antibody
Uniprot No.
Target Names
PHO1
Alternative Names
PHO1; At3g23430; MLM24.26; Phosphate transporter PHO1; Protein PHO1; AtPHO1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PHO1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Inositol polyphosphate sensor that associates with transcription factors to regulate inorganic phosphate (Pi) starvation responses. Probably acts by binding inositol polyphosphate via its SPX domain. Acts as a Pi exporter, mediating efflux of Pi out of cells. Transfers Pi from the epidermal and cortical cells to the root xylem vessels. Involved in the transfer of Pi from roots to shoots. Involved in abscisic acid (ABA) induction of stomatal closure and ABA repression of stomatal opening.
Gene References into Functions
  1. The Arabidopsis Pi exporters PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between the seed coat and embryo were analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1, or the pho1 phoh1 double mutant. PMID: 28943092
  2. Genome-wide association mapping identified PHOSPHATE 1 (PHO1) and genes such as Root System Architecture 1 (RSA1) associated with differences in root allometry, a highly plastic trait capturing the distribution of lateral roots along the primary axis. PMID: 23980140
  3. An important role of the EXS domain of PHO1 in a root-to-shoot signaling pathway linking phosphate deficiency response PMID: 26546667
  4. AtWRKY42 modulated Pi homeostasis by regulating the expression of PHO1 and PHT1;1 to adapt to environmental changes in inorganic phosphate (Pi) availability. PMID: 25733771
  5. PHR1 and PHO1 participate in the coregulation of Zn and Pi homeostasis. PMID: 24420568
  6. Expression of PHO1 in guard cells of Arabidopsis thaliana is required for full stomatal responses to abscisic acid. PMID: 22612335
  7. PHO1 can trigger Pi export in ectopic plant cells, strongly indicating that PHO1 is itself a Pi exporter. PMID: 22449068
  8. PHO1 over-expression in the shoot of Arabidopsis thaliana led to a two- to threefold increase in shoot inorganic phosphate content and a severe reduction in shoot growth. PMID: 21309867
  9. Reduction of PHO1 expression leads to wild-type growthof plants in phosphate-deficient media, despite Pi deficiency. PMID: 21288266
  10. Our results demonstrate that WRKY6 and WRKY42 are involved in Arabidopsis responses to low Pi stress by regulation of PHO1 expression. PMID: 19934380

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Subcellular Location
Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
SYG1 (TC 2.A.94) family
Tissue Specificity
Predominantly in roots, but also weak expression in the lower part of the hypocotyl. In the stellar cells, including the pericycle and xylem parenchyma cells, but not in the cortical or epidermal cells. Expressed in guard cells.
Database Links

KEGG: ath:AT3G23430

STRING: 3702.AT3G23430.1

UniGene: At.23989

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