pitx3 Antibody

Code CSB-PA750917XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) pitx3 Polyclonal antibody
Uniprot No.
Target Names
pitx3
Alternative Names
pitx3 antibody; si:dkey-196H17.1 antibody; Pituitary homeobox 3 antibody; Bicoid-like homeodomain transcription factor Pitx3 antibody; Homeobox protein PITX3 antibody; Paired-like homeodomain transcription factor 3 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) pitx3 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional regulator which may play a role in the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons. Required for lens and retinal development and for pituitary pre-placode formation and cell specification.
Gene References into Functions
  1. pitx3 expression in the primordia of the pituitary, lens, olfactory mucosa, and cranial ganglia is modulated by hedgehog and nodal proteins' signaling. PMID: 15645439
  2. expressing cells constitute an equivalence domain of cells that can form either pituitary or lens PMID: 15728669
  3. results demonstrate zebrafish pitx3 represents a true ortholog of the human PITX3 gene and the general function of the Pitx3 protein in lens development is conserved between mammals and the teleost fish PMID: 15804565
  4. Data demonstrate that Foxe3 is necessary for lens development in zebrafish and that foxe3 lies genetically downstream of pitx3 in a zebrafish lens development pathway. PMID: 16963235
  5. while lmx1b paralogues may contribute to the generation of diencephalic dopaminergic precursors. Conversely, knock-down of pitx3 does not specifically affect any diencephalic dopamine cluster PMID: 18053265

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
In the adult, high levels detected in the eye and much lower levels in internal organs such as liver, gastrointestinal system, heart, genitourinary system and pancreas.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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