Recombinant Danio rerio Pituitary homeobox 3 (pitx3)

Code CSB-YP750917DIL
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP750917DIL
MSDS
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Source E.coli
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Code CSB-EP750917DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750917DIL
MSDS
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Source Baculovirus
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Code CSB-MP750917DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pitx3
Uniprot No.
Alternative Names
pitx3; si:dkey-196H17.1; Pituitary homeobox 3; Bicoid-like homeodomain transcription factor Pitx3; Homeobox protein PITX3; Paired-like homeodomain transcription factor 3
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-293
Target Protein Sequence
MDFNLLTDSE ARSPALSLSD SGTPQHDPGC KGQDNSDTEK SHQNHTDESN PEDGSLKKKQ RRQRTHFTSQ QLQELEATFQ RNRYPDMSTR EEIAVWTNLT EARVRVWFKN RRAKWRKRER NQQAELCKNG FGAQFNGLMQ PYDDMYSGYS YNNWATKSLA SSPLSAKSFP FFNSMNVSPL SSQPMFSPPS SIPSMNMASS MVPSAVAGVP GSGLNNLGNL NNLNSPTLNS AAVSAAACPY ATTAGPYMYR DTCNSSLASL RLKAKQHANF AYPAVQNPVS NLSPCQYAVD RPV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator which may play a role in the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons. Required for lens and retinal development and for pituitary pre-placode formation and cell specification.
Gene References into Functions
  1. pitx3 expression in the primordia of the pituitary, lens, olfactory mucosa, and cranial ganglia is modulated by hedgehog and nodal proteins' signaling. PMID: 15645439
  2. expressing cells constitute an equivalence domain of cells that can form either pituitary or lens PMID: 15728669
  3. results demonstrate zebrafish pitx3 represents a true ortholog of the human PITX3 gene and the general function of the Pitx3 protein in lens development is conserved between mammals and the teleost fish PMID: 15804565
  4. Data demonstrate that Foxe3 is necessary for lens development in zebrafish and that foxe3 lies genetically downstream of pitx3 in a zebrafish lens development pathway. PMID: 16963235
  5. while lmx1b paralogues may contribute to the generation of diencephalic dopaminergic precursors. Conversely, knock-down of pitx3 does not specifically affect any diencephalic dopamine cluster PMID: 18053265

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
In the adult, high levels detected in the eye and much lower levels in internal organs such as liver, gastrointestinal system, heart, genitourinary system and pancreas.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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