pak-1 Antibody

Code CSB-PA619143XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans pak-1 Polyclonal antibody
Uniprot No.
Target Names
pak-1
Alternative Names
pak-1 antibody; C09B8.7 antibody; Serine/threonine-protein kinase pak-1 antibody; EC 2.7.11.1 antibody; CePAK antibody; p21-activated kinase 1 antibody; PAK1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans pak-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for hypodermal cell fusion, together with cdc-42 and ced-10, leading to embryonic body elongation, which involves dramatic cytoskeletal reorganization. Plays a redundant role with max-2 in dorsal axonal guidance in ventral cord commissural motoneurons and in P neuroblast migration. Acts probably downstream of Rho GTPases mig-2 and ced-10 to regulate these 2 processes. Involved in orientating axonal growth of HSN neurons. During gonad morphogenesis and probably in association with pix-1 and git-1, involved in the migration of distal tip cell (DTC) and in maintaining their sharp tapering morphology. In addition, plays a redundant role with max-2 in DTC-mediated guidance of gonad elongation. May phosphorylate mlc-4.
Gene References into Functions
  1. FOXO-Pak1 pathway was recently shown to regulate mammalian neuronal polarity, our findings indicate that the roles of FOXO and Pak1 in neuronal migration are most likely conserved from C. elegans to higher organisms. PMID: 23994474
  2. PAK1 promotes reproduction, whereas it inactivates HSP16.2 gene and shortens lifespan. PMID: 23524941
  3. Pak-1 interacts with Wnt signaling to regulate tissue polarity and gene expression. PMID: 23019370
  4. only PAK-1 functions in the GIT/PIX/PAK pathway independently of RAC/CDC42 GTPases. PMID: 19023419
Subcellular Location
Cell membrane; Peripheral membrane protein. Cytoplasm. Cell projection, axon. Perikaryon.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
Tissue Specificity
Specifically colocalized with cdc-42 and ced-10 at all hypodermal cell boundaries during embryo elongation throughout the second phase of embryogenesis. Expressed mainly in pharyngeal muscles, the CAN neurons, motor neurons in the ventral nerve cord, seve
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