Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) plo1 Polyclonal antibody

Uniprot No.

P50528

Target Names

plo1

Alternative Names

plo1 antibody; SPAC23C11.16 antibody; Serine/threonine-protein kinase plo1 antibody; EC 2.7.11.21 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) plo1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required to form a bipolar spindle, the actin ring and septum. Functions upstream of the whole septum formation pathway, including actin ring formation (regulated by late septation genes) and septal material deposition (regulated by early septation genes). Behaves as a 'septum-promoting factor', and could also be involved in inducing other late events of cell division. Together with moa1, acts as a regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage.

Gene References into Functions

The data presented in this study suggest there are at least two, genetically separable, states of the septation initiation network; an early state that is dependent upon Plo1p for its establishment, and a late state, which is dependent upon Spg1p. PMID: 25501816
Dam1 phosphorylation by Plo1 is dispensable for DASH assembly and chromosome retrieval but instead aids tension-dependent chromosome bi-orientation PMID: 22375062
Plo1 and a centrin play central roles in the meiotic spindle pole body remodeling PMID: 22438582
Plo1 facilitates contractile ring assembly at mitotic onset by regulating myosin II recruitment by Mid1/anillin. PMID: 21376600
Pdk1p functions in concert with Plo1p to regulate multiple processes such as the establishment of a bipolar mitotic spindle, transition to anaphase, placement of the actomyosin ring and proper execution of cytokinesis PMID: 15857958
phosphorylation of Plo1 Ser 402 not only enables stress response pathway signalling to modulate the timing of mitotic commitment in response to nutrient status in unperturbed cycles, but also promotes the return to normal cell cycle control after stress PMID: 15917811
Plo1p controls M-G1-phase gene transcription through a combination of phosphorylation and cell-cycle-specific DNA binding to Pombe cell cycle boxes. PMID: 18057023

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Subcellular Location

Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily

Database Links

KEGG: spo:SPAC23C11.16

STRING: 4896.SPAC23C11.16.1

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plo1 Antibody

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