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Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase plo1 (plo1), partial

Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase plo1 (plo1), partial

Code	CSB-YP342299SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP342299SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP342299SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP342299SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP342299SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

plo1

Uniprot No.

P50528

Alternative Names

plo1; SPAC23C11.16; Serine/threonine-protein kinase plo1; EC 2.7.11.21

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required to form a bipolar spindle, the actin ring and septum. Functions upstream of the whole septum formation pathway, including actin ring formation (regulated by late septation genes) and septal material deposition (regulated by early septation genes). Behaves as a 'septum-promoting factor', and could also be involved in inducing other late events of cell division. Together with moa1, acts as a regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage.

Gene References into Functions

The data presented in this study suggest there are at least two, genetically separable, states of the septation initiation network; an early state that is dependent upon Plo1p for its establishment, and a late state, which is dependent upon Spg1p. PMID: 25501816
Dam1 phosphorylation by Plo1 is dispensable for DASH assembly and chromosome retrieval but instead aids tension-dependent chromosome bi-orientation PMID: 22375062
Plo1 and a centrin play central roles in the meiotic spindle pole body remodeling PMID: 22438582
Plo1 facilitates contractile ring assembly at mitotic onset by regulating myosin II recruitment by Mid1/anillin. PMID: 21376600
Pdk1p functions in concert with Plo1p to regulate multiple processes such as the establishment of a bipolar mitotic spindle, transition to anaphase, placement of the actomyosin ring and proper execution of cytokinesis PMID: 15857958
phosphorylation of Plo1 Ser 402 not only enables stress response pathway signalling to modulate the timing of mitotic commitment in response to nutrient status in unperturbed cycles, but also promotes the return to normal cell cycle control after stress PMID: 15917811
Plo1p controls M-G1-phase gene transcription through a combination of phosphorylation and cell-cycle-specific DNA binding to Pombe cell cycle boxes. PMID: 18057023