Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans pmk-1 Polyclonal antibody

Uniprot No.

Q17446

Target Names

pmk-1

Alternative Names

pmk-1 antibody; B0218.3 antibody; Mitogen-activated protein kinase pmk-1 antibody; EC 2.7.11.24 antibody; Stress-activated protein kinase pmk-1 antibody; p38 MAP kinase 1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans pmk-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Serine/threonine kinase which responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Phosphorylates skn-1 which probably regulates skn-1 nuclear translocation in response to oxidative stress. Probably by activating skn-1, involved in the up-regulation of gcs-1 and glutathione-S-transferase gst-4 expression upon bacteria infection. Up-regulates expression of gcs-1 in intestinal cells upon arsenite treatment. Functions downstream of the MAPKK sek-1 and the MAPKKK nsy-1 as the MAP kinase which regulates pathogen resistance and responses to oxidative stress. Required for expression of antimicrobial peptide nlp-29 in response to fungal infection or physical injury. Involved in resistance to the nematotoxic C.cinerea galectin Cgl2. May play a redundant role with other MAP kinases in susceptibility to anoxia, downstream of tir-1/nsy-1. Phosphorylates transcription factor rnt-1 during oxidative stress which results in rnt-1 stabilization in the intestine. Phosphorylates transcription factor atf-7 during pathogen infection resulting in modulation of target genes. Probably downstream of nsy-1 and sek-1, involved in germline apoptosis induced by heavy metals, such as Cu(2+).

Gene References into Functions

The authors used RNAi to knock down p38 MAPK (pmk-1) expression. C. elegans MAPK mutants are hypersusceptible to mycobacterial infection and pathogenic mycobacteria inhibit MAPK-mediated protection through the MAPK phosphatase gene. PMID: 26919641
PMK-1 plays an important protective role in silver nanoparticle-induced oxidative DNA damage-repair. PMID: 24347047
the PMK-1 pathway is involved in the heat stress responses of C. elegans. PMID: 23117578
define the phenomenon of innate immunosenescence in C. elegans in terms of the age-dependent dynamics of the PMK-1 innate immune signaling pathway, and they suggest that a cycle of intestinal tissue aging, immunosenescence, and bacterial proliferation PMID: 21625567
These results establish a PMK-1-dependant PCD pathway as a C. elegans innate immune response to Salmonella PMID: 12526744
The contribution of the PMK-1 pathway to the enhanced lifespan of daf-2 mutants suggests that innate immunity is an important determinant of longevity. PMID: 17096597

Hide All

Subcellular Location

Nucleus.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Tissue Specificity

Expressed in intestinal cells.

Database Links

KEGG: cel:CELE_B0218.3

STRING: 6239.B0218.3

UniGene: Cel.12534

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA619138XA01CXY
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

pmk-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background