Pof Antibody

Code CSB-PA893358XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Pof Polyclonal antibody
Uniprot No.
Target Names
Pof
Alternative Names
Pof antibody; CG3691 antibody; Protein painting of fourth antibody; Zeste-interacting protein 16 antibody; Zip16 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Pof protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable RNA-binding protein that specifically binds to the fourth chromosome and may bind an RNA that spreads the fourth chromosome. May be a reminiscence of X chromosome dosage compensation of ancestral Drosophila species in which the X and the fourth chromosomes are one single chromosome.
Gene References into Functions
  1. The targeting of protein Painting of fourth to PoX1(POF-on-X) and PoX2 is largely dependent on roX expression and identified a high-affinity target region that ectopically recruits POF. PMID: 23733888
  2. POF binds to nascent RNA, and this binding is associated with increased transcription output from chromosome 4 PMID: 22473994
  3. POF is involved in a chromosome-specific regulatory function PMID: 15210994
Subcellular Location
Nucleus. Chromosome. Note=Specifically binds to the fourth chromosome.
Tissue Specificity
Weakly expressed in embryos. Expression increases during larval and pupal stages. In adults, it is predominantly expressed in males, while it is weakly expressed in females.
Database Links

KEGG: dme:Dmel_CG3691

STRING: 7227.FBpp0072234

UniGene: Dm.2706

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