Recombinant Drosophila melanogaster Protein painting of fourth (Pof)

Code CSB-YP893358DLU
MSDS
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Source Yeast
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Code CSB-EP893358DLU
MSDS
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Source E.coli
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Code CSB-EP893358DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893358DLU
MSDS
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Source Baculovirus
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Code CSB-MP893358DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pof
Uniprot No.
Alternative Names
Pof; CG3691; Protein painting of fourth; Zeste-interacting protein 16; Zip16
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-495
Target Protein Sequence
MDSKRAALES GDGPDAKRLD TTDDQDKEAS GGDGSQVMLA KHVAPYTGHG CTPPMESYLF EPTPAGSQLL PWKTSVDLDN DAELEKPTSD KKPDTAKLSR RELAKMRREH TLRALALERE LTNKPGQTPA SEVLLVRFPD PEITAPMLAG LSKDIRDVVL PISVAPRYCL VHLKAGADVE ATICDINRVR FGTGHLRAEL KPFSDEEQAE FIDPCSLYVG NIPFNMTTSA IKAYFANAMR VDIGVLKREK RARYAFVRYA SPDQTMEAFK ELVDSPLNSR TLTVRYRRLR KRAGMPMVQC ATSFQALQSP NGDDDNTDCK VISPPPLESI IISDSDNCSD SSGNGKEDGK RKKKINEQER EIEKLKRQMA EYGAIIKSLQ FRQNSLEDTF IPDLTPKVEP SVNPTGCLLG SNAVHLMRDI KKECDYLGIP DPVPATKPTT QAQDDSQKKA KRSCFGRLFT GPFRRGTSAM KTADEYEKDD RLEELYAQLE RDPDP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable RNA-binding protein that specifically binds to the fourth chromosome and may bind an RNA that spreads the fourth chromosome. May be a reminiscence of X chromosome dosage compensation of ancestral Drosophila species in which the X and the fourth chromosomes are one single chromosome.
Gene References into Functions
  1. The targeting of protein Painting of fourth to PoX1(POF-on-X) and PoX2 is largely dependent on roX expression and identified a high-affinity target region that ectopically recruits POF. PMID: 23733888
  2. POF binds to nascent RNA, and this binding is associated with increased transcription output from chromosome 4 PMID: 22473994
  3. POF is involved in a chromosome-specific regulatory function PMID: 15210994
Subcellular Location
Nucleus. Chromosome. Note=Specifically binds to the fourth chromosome.
Tissue Specificity
Weakly expressed in embryos. Expression increases during larval and pupal stages. In adults, it is predominantly expressed in males, while it is weakly expressed in females.
Database Links

KEGG: dme:Dmel_CG3691

STRING: 7227.FBpp0072234

UniGene: Dm.2706

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