ppiD Antibody

Code CSB-PA018474XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) ppiD Polyclonal antibody
Uniprot No.
Target Names
ppiD
Alternative Names
ppiD; ybaU; b0441; JW0431; Periplasmic chaperone PpiD; Periplasmic folding chaperone
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) ppiD protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Chaperone that functions as a gatekeeper on the periplasmic side of the SecYEG translocon. Facilitates the translocation of precursor proteins across SecYEG by interacting with the translocating substrate. Also plays a role in the release of newly synthesized secreted proteins at the periplasmic exit site of the Sec translocon. May be involved in the early periplasmic folding of many newly translocated proteins. Acts as a transient subunit of the Sec translocon that binds to the lateral gate of SecY and is detached by nascent membrane proteins but not by SecA.; Does not have peptidyl-prolyl isomerase (PPIase) activity. PPIase activity could not be generated by substitutions at the peptide binding site of the isolated parvulin-like domain.; (Microbial infection) May play an important role in bacteriophage T4 infection and be involved in the penetration of the inner membrane by the bacteriophage injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell.
Gene References into Functions
  1. PpiD contributes to the efficient detachment of newly secreted OmpA from the inner membrane and in doing so, seems to cooperate in a hierarchical manner with other periplasmic chaperones such as SurA, DegP, and Skp. PMID: 29097228
  2. PpiD functions as a chaperone and contributes to the network of periplasmic chaperone activities PMID: 20920237
  3. although PpiD and SurA have partially overlapping substrate specificities, they fulfil different functions in the cell PMID: 18498364
Subcellular Location
Cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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