PRE3 Antibody

Code CSB-PA880228XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PRE3 Polyclonal antibody
Uniprot No.
Target Names
PRE3
Alternative Names
PRE3 antibody; BHLH135 antibody; BS1 antibody; EN67 antibody; TMO7 antibody; At1g74500 antibody; F1M20.18 antibody; Transcription factor PRE3 antibody; Basic helix-loop-helix protein 135 antibody; AtbHLH135 antibody; bHLH 135 antibody; Protein ACTIVATION-TAGGED BRI1 SUPPRESSOR 1 antibody; ATBS1 antibody; Protein PACLOBUTRAZOL RESISTANCE 3 antibody; Protein TARGET OF MOOPTEROS 7 antibody; Transcription factor EN 67 antibody; bHLH transcription factor bHLH135 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PRE3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Atypical and probable non DNA-binding bHLH transcription factor required for MONOPTEROS-dependent root initiation in embryo. Promotes the correct definition of the hypophysis cell division plane. Transcriptionally controlled by MONOPTEROS. Moves from its site of synthesis in pro-embryos cells into the hypophysis. Regulates brassinosteroid (BR) signaling by sequestering negative BR signaling components. May function as positive regulator of gibberellin signaling. May play a role in the regulation of light signaling and possibly auxin signaling.
Gene References into Functions
  1. In the post-embryonic root, TMO7 and its close relatives are transported into the root cap through plasmodesmata in a sequence-dependent manner. Nuclear residence is crucial for TMO7 transport, and we postulate that modification, potentially phosphorylation, labels TMO7 for transport. PMID: 29358212
  2. TMO7 is involved in the regulation of light signaling. PMID: 22339648
  3. TARGET OF MP, TMO5 and TMO7 encode basic helix-loop-helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MONOPTEROS-dependent root initiation. [TMO7] PMID: 20220754
  4. Plants use atypical Basic helix-loop-helix (bHLH) proteins to regulate BR signaling. [ATBS1] PMID: 20023194
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Expressed in root and shoot meristems, and young siliques. Low levels detected in all aerial tissues.
Database Links

KEGG: ath:AT1G74500

STRING: 3702.AT1G74500.1

UniGene: At.43100

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