Recombinant Arabidopsis thaliana Transcription factor bHLH135 (BHLH135)

Code CSB-YP880228DOA
MSDS
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Source Yeast
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Code CSB-EP880228DOA
MSDS
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Source E.coli
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Code CSB-EP880228DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880228DOA
MSDS
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Source Baculovirus
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Code CSB-MP880228DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRE3
Uniprot No.
Alternative Names
PRE3; BHLH135; BS1; EN67; TMO7; At1g74500; F1M20.18; Transcription factor PRE3; Basic helix-loop-helix protein 135; AtbHLH135; bHLH 135; Protein ACTIVATION-TAGGED BRI1 SUPPRESSOR 1; ATBS1; Protein PACLOBUTRAZOL RESISTANCE 3; Protein TARGET OF MOOPTEROS 7; Transcription factor EN 67; bHLH transcription factor bHLH135
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-93
Target Protein Sequence
MSGRRSRSRQ SSGTSRISED QINDLIIKLQ QLLPELRDSR RSDKVSAARV LQDTCNYIRN LHREVDDLSE RLSELLANSD TAQAALIRSL LTQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Atypical and probable non DNA-binding bHLH transcription factor required for MONOPTEROS-dependent root initiation in embryo. Promotes the correct definition of the hypophysis cell division plane. Transcriptionally controlled by MONOPTEROS. Moves from its site of synthesis in pro-embryos cells into the hypophysis. Regulates brassinosteroid (BR) signaling by sequestering negative BR signaling components. May function as positive regulator of gibberellin signaling. May play a role in the regulation of light signaling and possibly auxin signaling.
Gene References into Functions
  1. In the post-embryonic root, TMO7 and its close relatives are transported into the root cap through plasmodesmata in a sequence-dependent manner. Nuclear residence is crucial for TMO7 transport, and we postulate that modification, potentially phosphorylation, labels TMO7 for transport. PMID: 29358212
  2. TMO7 is involved in the regulation of light signaling. PMID: 22339648
  3. TARGET OF MP, TMO5 and TMO7 encode basic helix-loop-helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MONOPTEROS-dependent root initiation. [TMO7] PMID: 20220754
  4. Plants use atypical Basic helix-loop-helix (bHLH) proteins to regulate BR signaling. [ATBS1] PMID: 20023194
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Expressed in root and shoot meristems, and young siliques. Low levels detected in all aerial tissues.
Database Links

KEGG: ath:AT1G74500

STRING: 3702.AT1G74500.1

UniGene: At.43100

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