Prim1 Antibody

Rare Species
Code CSB-PA630508XA01DLU
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes: Prim1 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 33 kDa
    Observed band size: 33 kDa
Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Uniprot No.
Target Names
Alternative Names
DNA primase small subunit (EC 2.7.7.-) (DNA polymerase subunit A) (DNA primase 50 kDa subunit) (dPRI50) DNApol-alpha50 Pri50 CG7108
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Prim1 protein (181-438aa)
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The Prim1 polyclonal antibody is raised in a rabbit immunized with a recombinant Drosophila melanogaster (Fruit fly) Prim1 protein (amino acids 181-438). Following several repeated immunizations, the rabbit serum is collected and then subjected to affinity chromatography purification to obtain the polyclonal antibodies against Prim1. The functionality of the Prim1 antibody is established through ELISA and WB assays, affirming its proficiency in detecting the Drosophila melanogaster (Fruit fly) Prim1 protein in experimental settings.

The Drosophila melanogaster Prim1 protein is a member of the DNA primase family of proteins. It is responsible for the synthesis of the RNA primer that is used to initiate DNA replication and is essential for DNA replication in Drosophila melanogaster. The Prim1 protein is a heterodimer that consists of a catalytic subunit and a regulatory subunit. The catalytic subunit contains the active site for RNA synthesis, while the regulatory subunit is responsible for binding to DNA and regulating the activity of the catalytic subunit.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit PolA1, an accessory subunit PolA2 and two primase subunits, the catalytic subunit Prim1 and the regulatory subunit Prim2) is recruited to DNA at the replicative forks. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit. Can add both ribo- and deoxynucleotides during elongation of the primers. Binds single stranded DNA.
Protein Families
Eukaryotic-type primase small subunit family
Tissue Specificity
Expressed in embryos (at protein level).
Database Links

KEGG: dme:Dmel_CG7108

STRING: 7227.FBpp0076330

UniGene: Dm.6051

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1