PRK6 Antibody

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Code	CSB-PA662980XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PRK6 Polyclonal antibody

Uniprot No.

Q3E991

Target Names

PRK6

Alternative Names

PRK6 antibody; At5g20690 antibody; T1M15.90 antibody; Pollen receptor-like kinase 6 antibody; AtPRK6 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PRK6 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Key receptor for sensing species-specific attractants in cooperation with other pollen receptor-like kinases. Essential for pollen tube reorientation toward attractant peptides.

Gene References into Functions

Arabidopsis pollen receptor like kinase 6 (AtPRK6) is localized to the plasma membrane and expressed specifically in pollen tubes. The interactions between AtPRK6 and Arabidopsis Rop guanine nucleotide exchange factors AtRopGEF8 and AtRopGEF12 are mediated by the C-termini of AtRopGEF8/12 and by the juxtamembrane and kinase domain of AtPRK6, but are not dependent on the kinase activity. PMID: 28795376
Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6(LRR)) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. PMID: 29109411
PRK6 acts as a key membrane receptor for external AtLURE1 attractants, and recruits the core tip-growth machinery, including ROP signalling proteins. [PRK6] PMID: 26961657

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasmic granule.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Expressed specifically in the pollen tube, predominantly at the tip.

Database Links

KEGG: ath:AT5G20690

STRING: 3702.AT5G20690.1

UniGene: At.54932

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Service

Phage Display Service

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Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PRK6 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background