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Recombinant Arabidopsis thaliana Probable inactive leucine-rich repeat receptor-like protein kinase At5g20690 (At5g20690), partial

Recombinant Arabidopsis thaliana Probable inactive leucine-rich repeat receptor-like protein kinase At5g20690 (At5g20690), partial

Code	CSB-YP662980DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP662980DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP662980DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP662980DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP662980DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRK6

Uniprot No.

Q3E991

Alternative Names

PRK6; At5g20690; T1M15.90; Pollen receptor-like kinase 6; AtPRK6

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Key receptor for sensing species-specific attractants in cooperation with other pollen receptor-like kinases. Essential for pollen tube reorientation toward attractant peptides.

Gene References into Functions

Arabidopsis pollen receptor like kinase 6 (AtPRK6) is localized to the plasma membrane and expressed specifically in pollen tubes. The interactions between AtPRK6 and Arabidopsis Rop guanine nucleotide exchange factors AtRopGEF8 and AtRopGEF12 are mediated by the C-termini of AtRopGEF8/12 and by the juxtamembrane and kinase domain of AtPRK6, but are not dependent on the kinase activity. PMID: 28795376
Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6(LRR)) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. PMID: 29109411
PRK6 acts as a key membrane receptor for external AtLURE1 attractants, and recruits the core tip-growth machinery, including ROP signalling proteins. [PRK6] PMID: 26961657

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasmic granule.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Expressed specifically in the pollen tube, predominantly at the tip.

Database Links

KEGG: ath:AT5G20690

STRING: 3702.AT5G20690.1

UniGene: At.54932

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