PRF1 Antibody

Code CSB-PA677077XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PRF1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
PRF1 antibody; PFN1 antibody; PRO1 antibody; At2g19760 antibody; F6F22.21 antibody; Profilin-1 antibody; AtPROF1 antibody; AthPRF1 antibody; allergen Ara t 8 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PRF1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Binds to actin monomers and regulates the organization of the actin cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. At low concentrations, associates with the poly-proline motif of formins to enhance actin filament elongation rate. Binds ACT1, ACT7 and ACT11 and inhibits actin polymerization. Coordinates the stochastic dynamic properties of actin filaments by modulating formin-mediated actin nucleation and assembly during axial cell expansion. Binds G-actin and poly-L-proline in vitro. Inhibits cell growth of various pathogenic fungal strains. May play a role as antifungal proteins in the defense system against fungal pathogen attacks.
Gene References into Functions
  1. PRF1 coordinates the stochastic dynamic properties of actin filaments by modulating formin-mediated actin nucleation and assembly during plant cell expansion. PMID: 26574597
  2. formed a filamentous network likely associated with actin filaments PMID: 19200149
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm.
Protein Families
Profilin family
Tissue Specificity
Expressed at low levels roots, leaves, stems, flowers and siliques. Expressed in leaf epidermal cells, trichomes and stem epidermal cells. Detected in phloem exudates (at protein level).
Database Links

KEGG: ath:AT2G19760

STRING: 3702.AT2G19760.1

UniGene: At.23712

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