PSBS Antibody

Code CSB-PA894502XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PSBS Polyclonal antibody
Uniprot No.
Target Names
PSBS
Alternative Names
PSBS; NPQ4; At1g44575; T18F15.3; Photosystem II 22 kDa protein, chloroplastic; CP22; Protein NONPHOTOCHEMICAL QUENCHING 4; Protein PHOTOSYSTEM II SUBUNIT S
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PSBS protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays an important role in non-photochemical quenching, a process maintains the balance between dissipation and utilization of light energy to minimize generation of oxidizing molecules, thereby protecting the plant against photo-oxidative damage. Is not necessary for efficient light harvesting and photosynthesis.
Gene References into Functions
  1. The interaction of PsbS with the antenna system is affected by both DeltapH and the level of zeaxanthin. PMID: 28134919
  2. the importance of LHCII aggregation in the NPQ mechanism PMID: 26233261
  3. Npq1 and npq4 mutants are equally protected against photodamage, but less protected than WT plants. PMID: 25429003
  4. It was concluded that functional PsbS is required for optimization of absorbed photon fate and optimal light acclimatory responses. PMID: 25654166
  5. Leaves from three different Arabidopsis lines with different expression levels of PsbS protein showed different levels of non-photochemical quenching. The PsbS deficient plant npq4 showed remarkable reduction of electron transport rate. PMID: 25047739
  6. the presence of violaxanthin deepoxidase, but not the protein PsbS, changes the excited-state relaxation dynamics of chlorophyll; the presence of both zeaxanthin and PsbS is required for strong quenching PMID: 25422428
  7. Changes in the complement of light-harvesting complexes and PsbS have striking effects on the photosystem II macrostructure, and these effects correlate with changes in the mobility of chlorophyll proteins within the thylakoid membrane. PMID: 21919982
  8. Direct interaction of the major light-harvesting complex II and PsbS in nonphotochemical quenching. PMID: 23509270
  9. Restoration of rapidly reversible photoprotective energy dissipation in the absence of PsbS protein by enhanced DeltapH. PMID: 21474447
  10. Data suggest that PsbS plays the role of a kinetic modulator of the energy dissipation process in the PSII light-harvesting antenna. PMID: 19843315
  11. Data suggest that PsbS controls the macro-organisation of the grana membrane, providing an explanation of its role in NPQ. PMID: 20035752
  12. The effects of two different mutations of PsbS on the metabolism of photosystem II in A. thaliana are reported. PMID: 16075321
  13. Binding of two molecules of zeaxanthin in the three-dimensional model of PsbS is postulated. The molecular mechanism of photoprotection by PsbS is explained through the model. PMID: 16538483
  14. PsbS acts as a trigger of the conformational change that leads to the establishment of nonphotochemical quenching. PMID: 16545380
  15. Transgenic Arabidopsis lines synthesizing only the altered psbS forms exhibited severely impaired NPQ capacity PMID: 17306227
  16. xanthophyll dependence of energy dissipation is not due to PsbS but to other pigment-binding proteins, probably of the Lhcb type PMID: 18070876
  17. The level of PsbS is a determinant of plant fitness, metabolism and gene expression. Lack of PsbS results in increased photooxidative stress in the chloroplasts. PMID: 19171025

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Subcellular Location
Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein.
Protein Families
ELIP/psbS family
Database Links

KEGG: ath:AT1G44575

STRING: 3702.AT1G44575.1

UniGene: At.21534

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