Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ptsG Polyclonal antibody

Uniprot No.

P69786

Target Names

ptsG

Alternative Names

ptsG antibody; glcA antibody; umg antibody; b1101 antibody; JW1087 antibody; PTS system glucose-specific EIICB component antibody; EIICB-Glc antibody; EII-Glc) [Includes: Glucose permease IIC component antibody; PTS system glucose-specific EIIC component); Glucose-specific phosphotransferase enzyme IIB component antibody; EC 2.7.1.199 antibody; PTS system glucose-specific EIIB component)] antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) ptsG protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport. Also functions as a chemoreceptor monitoring the environment for changes in sugar concentration and an effector modulating the activity of the transcriptional repressor Mlc.; (Microbial infection) Probably transports the toxic C-terminal region of CdiA from E.coli strains NC101 and 3006 across the inner membrane to the cytoplasm, where CdiA degrades specific tRNAs. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins. Probably also serves as the inner membrane receptor for CdiA-STECO31 from E.coli strain STEC_O31 (Probable).

Gene References into Functions

SgrT acts to specifically inhibit the transport activity of the major glucose permease PtsG. PMID: 28289085
This work presents the structure of a glucose PTS transporter IICB at the highest resolution achieved so far and sets the basis for future structural studies. PMID: 21996078
crystallization of the tryptic EIIC(Glc) domain is described. A complete data set was collected to 4.5 A resolution at 100 K. PMID: 20516600
SgrS 168-181 is a minimal base-pairing region for translational inhibition of ptsG mRNA. PMID: 20345651
ptsG activates glucose transport under oxidative stress conditions, and is regulated by the SoxRS system. PMID: 18368388

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW1087

STRING: 316385.ECDH10B_1173

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ptsG Antibody

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