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Recombinant Escherichia coli PTS system glucose-specific EIICB component (ptsG), partial

Recombinant Escherichia coli PTS system glucose-specific EIICB component (ptsG), partial

Code	CSB-YP303193ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP303193ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP303193ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP303193ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP303193ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ptsG

Uniprot No.

P69786

Alternative Names

ptsG; glcA; umg; b1101; JW1087; PTS system glucose-specific EIICB component; EIICB-Glc; EII-Glc) [Includes: Glucose permease IIC component; PTS system glucose-specific EIIC component); Glucose-specific phosphotransferase enzyme IIB component; EC 2.7.1.199; PTS system glucose-specific EIIB component)]

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport. Also functions as a chemoreceptor monitoring the environment for changes in sugar concentration and an effector modulating the activity of the transcriptional repressor Mlc.; (Microbial infection) Probably transports the toxic C-terminal region of CdiA from E.coli strains NC101 and 3006 across the inner membrane to the cytoplasm, where CdiA degrades specific tRNAs. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins. Probably also serves as the inner membrane receptor for CdiA-STECO31 from E.coli strain STEC_O31 (Probable).

Gene References into Functions

SgrT acts to specifically inhibit the transport activity of the major glucose permease PtsG. PMID: 28289085
This work presents the structure of a glucose PTS transporter IICB at the highest resolution achieved so far and sets the basis for future structural studies. PMID: 21996078
crystallization of the tryptic EIIC(Glc) domain is described. A complete data set was collected to 4.5 A resolution at 100 K. PMID: 20516600
SgrS 168-181 is a minimal base-pairing region for translational inhibition of ptsG mRNA. PMID: 20345651
ptsG activates glucose transport under oxidative stress conditions, and is regulated by the SoxRS system. PMID: 18368388

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW1087

STRING: 316385.ECDH10B_1173

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