NPF7.3 Antibody

Code CSB-PA873316XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NPF7.3 Polyclonal antibody
Uniprot No.
Target Names
NPF7.3
Alternative Names
NPF7.3 antibody; NRT1.5 antibody; At1g32450 antibody; F5D14.23 antibody; Protein NRT1/ PTR FAMILY 7.3 antibody; AtNPF7.3 antibody; Nitrate transporter 1.5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NPF7.3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Low-affinity proton-dependent bidirectional nitrate transporter. Involved in nitrate loading into xylem and not in nitrate uptake. Not involved in histidine or dipeptides transport.
Gene References into Functions
  1. This study reveals that NRT1.5 plays a crucial role in K(+) translocation from root to shoot and is also involved in the coordination of potassium/nitrates distribution in plants. PMID: 28739644
  2. a possible involvement of NPF7.3/NRT1.5 in auxin homeostasis in roots under potassium deprivation, is reported. PMID: 27089248
  3. NRT1.5 likely perceives nitrate starvation-derived signals to prevent leaf senescence by facilitating foliar potassium accumulation PMID: 26732494
  4. NRT1.5 is involved in nitrate allocation to roots and the consequent tolerance to several stresses. PMID: 22685171
  5. NRT1.5 participates in root xylem loading of nitrate but in addition to the pathway involving NRT1.5, another mechanism is responsible for xylem loading of nitrate. [NRT1.5] PMID: 18780802

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
PTR2/POT transporter (TC 2.A.17) family
Tissue Specificity
High expression in roots. Barely detected in shoots. Expressed in root pericycle cells close to the xylem.
Database Links

KEGG: ath:AT1G32450

STRING: 3702.AT1G32450.1

UniGene: At.43846

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