Recombinant Arabidopsis thaliana Nitrate transporter 1.5 (NRT1.5), partial

Code CSB-YP873316DOA
MSDS
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Source Yeast
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Code CSB-EP873316DOA
MSDS
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Source E.coli
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Code CSB-EP873316DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873316DOA
MSDS
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Source Baculovirus
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Code CSB-MP873316DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NPF7.3
Uniprot No.
Alternative Names
NPF7.3; NRT1.5; At1g32450; F5D14.23; Protein NRT1/ PTR FAMILY 7.3; AtNPF7.3; Nitrate transporter 1.5
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Low-affinity proton-dependent bidirectional nitrate transporter. Involved in nitrate loading into xylem and not in nitrate uptake. Not involved in histidine or dipeptides transport.
Gene References into Functions
  1. This study reveals that NRT1.5 plays a crucial role in K(+) translocation from root to shoot and is also involved in the coordination of potassium/nitrates distribution in plants. PMID: 28739644
  2. a possible involvement of NPF7.3/NRT1.5 in auxin homeostasis in roots under potassium deprivation, is reported. PMID: 27089248
  3. NRT1.5 likely perceives nitrate starvation-derived signals to prevent leaf senescence by facilitating foliar potassium accumulation PMID: 26732494
  4. NRT1.5 is involved in nitrate allocation to roots and the consequent tolerance to several stresses. PMID: 22685171
  5. NRT1.5 participates in root xylem loading of nitrate but in addition to the pathway involving NRT1.5, another mechanism is responsible for xylem loading of nitrate. [NRT1.5] PMID: 18780802

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
PTR2/POT transporter (TC 2.A.17) family
Tissue Specificity
High expression in roots. Barely detected in shoots. Expressed in root pericycle cells close to the xylem.
Database Links

KEGG: ath:AT1G32450

STRING: 3702.AT1G32450.1

UniGene: At.43846

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