Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NPF2.10 Polyclonal antibody

Uniprot No.

Q944G5

Target Names

NPF2.10

Alternative Names

NPF2.10 antibody; GTR1 antibody; At3g47960 antibody; T17F15.170 antibody; Protein NRT1/ PTR FAMILY 2.10 antibody; AtNPF2.10 antibody; Protein GLUCOSINOLATE TRANSPORTER-1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NPF2.10 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

High-affinity, proton-dependent glucosinolate-specific transporter. Involved in the distribution of glucosinolates within the leaf, including import into the glucosinolate-rich S-cells located adjacent to the phloem. Involved in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots.

Gene References into Functions

Expression of glucosinolate (GLS) importers GTR1 and GTR2 inside the stele, along with earlier observations, suggest three possible routes of stele-synthesized aliphatic GLS after their synthesis: (i) GTR-dependent import to cells symplastically connected to cortical cells and rhizosphere; (ii) GTR-independent transport via xylem to the shoot; and (iii) GTR-dependent import to GLS-degrading myrosin cells at the cortex. PMID: 27702989
dimerization of GTR1, possibly induced by dephosphorylation of the Thr residue located between the possible transmembrane regions, regulates its plasma membrane localization. PMID: 28594299
GTR1 would be involved in the translocation of JA and JA-Ile in plant and may be contributed to correct positioning of JA and JA-Ile to attenuate an excessive wound response in undamaged leaves PMID: 27760496
Phosphorylation of AtGTR1 is not directly involved in regulating AtGTR1 transport activity. PMID: 26340317
GTR1 role of transport processes in leaf glucosinolate distribution PMID: 25209984
glucosinolate can specifically translocate from rosette and roots to inflorescence in a GTR1- and GTR2-dependent manner PMID: 24481282
GTR1 and GTR2 control the loading of glucosinolates from the apoplasm into the phloem. [GTR1] PMID: 22864417

Hide All

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

PTR2/POT transporter (TC 2.A.17) family

Tissue Specificity

Expressed in stems, flowers, siliques, roots, shoots and leaves. Expressed in veins and in adjacent mesophyll cells in leaves, and in the root vasculature with highest expression in lateral branching points.

Database Links

KEGG: ath:AT3G47960

STRING: 3702.AT3G47960.1

UniGene: At.20263

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NPF2.10 Antibody

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