Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At3g47960 (At3g47960), partial

Code CSB-YP821983DOA
MSDS
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Source Yeast
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Code CSB-EP821983DOA
MSDS
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Source E.coli
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Code CSB-EP821983DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP821983DOA
MSDS
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Source Baculovirus
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Code CSB-MP821983DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NPF2.10
Uniprot No.
Alternative Names
NPF2.10; GTR1; At3g47960; T17F15.170; Protein NRT1/ PTR FAMILY 2.10; AtNPF2.10; Protein GLUCOSINOLATE TRANSPORTER-1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
High-affinity, proton-dependent glucosinolate-specific transporter. Involved in the distribution of glucosinolates within the leaf, including import into the glucosinolate-rich S-cells located adjacent to the phloem. Involved in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots.
Gene References into Functions
  1. Expression of glucosinolate (GLS) importers GTR1 and GTR2 inside the stele, along with earlier observations, suggest three possible routes of stele-synthesized aliphatic GLS after their synthesis: (i) GTR-dependent import to cells symplastically connected to cortical cells and rhizosphere; (ii) GTR-independent transport via xylem to the shoot; and (iii) GTR-dependent import to GLS-degrading myrosin cells at the cortex. PMID: 27702989
  2. dimerization of GTR1, possibly induced by dephosphorylation of the Thr residue located between the possible transmembrane regions, regulates its plasma membrane localization. PMID: 28594299
  3. GTR1 would be involved in the translocation of JA and JA-Ile in plant and may be contributed to correct positioning of JA and JA-Ile to attenuate an excessive wound response in undamaged leaves PMID: 27760496
  4. Phosphorylation of AtGTR1 is not directly involved in regulating AtGTR1 transport activity. PMID: 26340317
  5. GTR1 role of transport processes in leaf glucosinolate distribution PMID: 25209984
  6. glucosinolate can specifically translocate from rosette and roots to inflorescence in a GTR1- and GTR2-dependent manner PMID: 24481282
  7. GTR1 and GTR2 control the loading of glucosinolates from the apoplasm into the phloem. [GTR1] PMID: 22864417

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
PTR2/POT transporter (TC 2.A.17) family
Tissue Specificity
Expressed in stems, flowers, siliques, roots, shoots and leaves. Expressed in veins and in adjacent mesophyll cells in leaves, and in the root vasculature with highest expression in lateral branching points.
Database Links

KEGG: ath:AT3G47960

STRING: 3702.AT3G47960.1

UniGene: At.20263

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