PUB1 Antibody

Code CSB-PA339279XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PUB1 Polyclonal antibody
Uniprot No.
Target Names
PUB1
Alternative Names
PUB1 antibody; RNP1 antibody; YNL016W antibody; N2842 antibody; Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 antibody; ARS consensus-binding protein ACBP-60 antibody; Poly uridylate-binding protein antibody; Poly(U)-binding protein antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PUB1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May be associated with hnRNA within the nucleus and remains associated during nucleocytoplasmic mRNA transport, once the proteins are in the cytoplasm, disassembly of PUB1-RNA complexes may occur prior to PAB1 binding and formation of a translationally competent RNP complex. Binds to polyadenylated RNA; prefers to bind poly(rU); binds to T-rich single-stranded DNA.
Gene References into Functions
  1. Mutagenesis analysis revealed that residues on the beta-sheets of Pub1RRM1 and Pub1RRM2 are critical for poly(U) binding. PMID: 20438847
  2. Global analysis of Pub1 targets reveals a coordinate control of gene expression through modulation of binding and stability. PMID: 15964806
  3. PUB1 functions together with NAB2 to modulate mRNA stability and strengthen a model where nuclear events are coupled to the control of mRNA in the cytoplasm. PMID: 17636033
  4. The second of three RNA-recognition motifs (RRM) of Pub1 has been crystallized in this study; the second RRM may play a central role in poly(U) binding and determine the binding specificity of Pub1. PMID: 19193997
Subcellular Location
Cytoplasm. Nucleus.
Database Links

KEGG: sce:YNL016W

STRING: 4932.YNL016W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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