Recombinant Saccharomyces cerevisiae Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 (PUB1)

Code CSB-YP339279SVG
MSDS
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Source Yeast
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Code CSB-EP339279SVG
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Source E.coli
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Code CSB-EP339279SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339279SVG
MSDS
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Source Baculovirus
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Code CSB-MP339279SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PUB1
Uniprot No.
Alternative Names
PUB1; RNP1; YNL016W; N2842; Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1; ARS consensus-binding protein ACBP-60; Poly uridylate-binding protein; Poly(U)-binding protein
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-453
Target Protein Sequence
SENNEEQHQ QQQQQQPVAV ETPSAVEAPA SADPSSEQSV AVEGNSEQAE DNQGENDPSV VPANAITGGR ETSDRVLYVG NLDKAITEDI LKQYFQVGGP IANIKIMIDK NNKNVNYAFV EYHQSHDANI ALQTLNGKQI ENNIVKINWA FQSQQSSSDD TFNLFVGDLN VNVDDETLRN AFKDFPSYLS GHVMWDMQTG SSRGYGFVSF TSQDDAQNAM DSMQGQDLNG RPLRINWAAK RDNNNNNNYQ QRRNYGNNNR GGFRQYNSNN NNNMNMGMNM NMNMNMNNSR GMPPSSMGMP IGAMPLPSQG QPQQSQTIGL PPQVNPQAVD HIIRSAPPRV TTAYIGNIPH FATEADLIPL FQNFGFILDF KHYPEKGCCF IKYDTHEQAA VCIVALANFP FQGRNLRTGW GKERSNFMPQ QQQQGGQPLI MNDQQQPVMS EQQQQQQQQQ QQQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be associated with hnRNA within the nucleus and remains associated during nucleocytoplasmic mRNA transport, once the proteins are in the cytoplasm, disassembly of PUB1-RNA complexes may occur prior to PAB1 binding and formation of a translationally competent RNP complex. Binds to polyadenylated RNA; prefers to bind poly(rU); binds to T-rich single-stranded DNA.
Gene References into Functions
  1. Mutagenesis analysis revealed that residues on the beta-sheets of Pub1RRM1 and Pub1RRM2 are critical for poly(U) binding. PMID: 20438847
  2. Global analysis of Pub1 targets reveals a coordinate control of gene expression through modulation of binding and stability. PMID: 15964806
  3. PUB1 functions together with NAB2 to modulate mRNA stability and strengthen a model where nuclear events are coupled to the control of mRNA in the cytoplasm. PMID: 17636033
  4. The second of three RNA-recognition motifs (RRM) of Pub1 has been crystallized in this study; the second RRM may play a central role in poly(U) binding and determine the binding specificity of Pub1. PMID: 19193997
Subcellular Location
Cytoplasm. Nucleus.
Database Links

KEGG: sce:YNL016W

STRING: 4932.YNL016W

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