PYL9 Antibody

Code CSB-PA772824XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PYL9 Polyclonal antibody
Uniprot No.
Target Names
PYL9
Alternative Names
PYL9 antibody; RCAR1 antibody; At1g01360 antibody; F6F3.16 antibody; Abscisic acid receptor PYL9 antibody; ABI1-binding protein 4 antibody; PYR1-like protein 9 antibody; Regulatory components of ABA receptor 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PYL9 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) in an ABA-independent manner but more efficiently when activated by ABA. Confers enhanced sensitivity to ABA. Can be activated only by (+)-ABA but not by (-)-ABA.
Gene References into Functions
  1. AtRAE1 likely participates in ABA signalling through regulating the degradation of abscissic acid receptor RCAR1. PMID: 29044697
  2. Results show that PYL9 not just binds to MYB transcription factors, but also regulates their transcriptional activity, and that this is a point of convergence between abscisic acid and auxin pathways. PMID: 27256015
  3. results uncover the molecular mechanism of ABA-induced leaf senescence and suggest an important role of PYL9 and leaf senescence in promoting resistance to extreme drought stress PMID: 26831097
  4. Structural and binding studies of PYL9 reveals that the nonpolar triplet (Ile110, Val162, and Leu165) and Pro64 contribute to enhance ABA-binding affinity by inducing a shift of the ABA carboxyl group to form additional direct bonds with Asn169. PMID: 24645846
  5. RCAR receptor contributes to ozone, reduced air humidity and darkness induced stomatal regulation. PMID: 23703845

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Subcellular Location
Cytoplasm. Nucleus. Cell membrane.
Protein Families
PYR/PYL/RCAR abscisic acid intracellular receptor family
Tissue Specificity
Expressed in root tips, vascular tissues, stomata, flowers, pollen tubes and developing seeds.
Database Links

KEGG: ath:AT1G01360

STRING: 3702.AT1G01360.1

UniGene: At.42653

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