Recombinant Arabidopsis thaliana Abscisic acid receptor PYL9 (PYL9)

Code CSB-YP772824DOA
MSDS
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Source Yeast
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Code CSB-EP772824DOA
MSDS
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Source E.coli
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Code CSB-EP772824DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP772824DOA
MSDS
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Source Baculovirus
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Code CSB-MP772824DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PYL9
Uniprot No.
Alternative Names
PYL9; RCAR1; At1g01360; F6F3.16; Abscisic acid receptor PYL9; ABI1-binding protein 4; PYR1-like protein 9; Regulatory components of ABA receptor 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-187
Target Protein Sequence
MMDGVEGGTA MYGGLETVQY VRTHHQHLCR ENQCTSALVK HIKAPLHLVW SLVRRFDQPQ KYKPFVSRCT VIGDPEIGSL REVNVKSGLP ATTSTERLEL LDDEEHILGI KIIGGDHRLK NYSSILTVHP EIIEGRAGTM VIESFVVDVP QGNTKDETCY FVEALIRCNL KSLADVSERL ASQDITQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) in an ABA-independent manner but more efficiently when activated by ABA. Confers enhanced sensitivity to ABA. Can be activated only by (+)-ABA but not by (-)-ABA.
Gene References into Functions
  1. AtRAE1 likely participates in ABA signalling through regulating the degradation of abscissic acid receptor RCAR1. PMID: 29044697
  2. Results show that PYL9 not just binds to MYB transcription factors, but also regulates their transcriptional activity, and that this is a point of convergence between abscisic acid and auxin pathways. PMID: 27256015
  3. results uncover the molecular mechanism of ABA-induced leaf senescence and suggest an important role of PYL9 and leaf senescence in promoting resistance to extreme drought stress PMID: 26831097
  4. Structural and binding studies of PYL9 reveals that the nonpolar triplet (Ile110, Val162, and Leu165) and Pro64 contribute to enhance ABA-binding affinity by inducing a shift of the ABA carboxyl group to form additional direct bonds with Asn169. PMID: 24645846
  5. RCAR receptor contributes to ozone, reduced air humidity and darkness induced stomatal regulation. PMID: 23703845

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Subcellular Location
Cytoplasm. Nucleus. Cell membrane.
Protein Families
PYR/PYL/RCAR abscisic acid intracellular receptor family
Tissue Specificity
Expressed in root tips, vascular tissues, stomata, flowers, pollen tubes and developing seeds.
Database Links

KEGG: ath:AT1G01360

STRING: 3702.AT1G01360.1

UniGene: At.42653

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