RAD59 Antibody

Code CSB-PA619572XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD59 Polyclonal antibody
Uniprot No.
Target Names
RAD59
Alternative Names
RAD59 antibody; YDL059C antibody; DNA repair protein RAD59 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD59 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing. Anneals complementary single-stranded DNA.
Gene References into Functions
  1. propose that Rad59 promotes fork progression when Okazaki fragment processing is compromised and counteracts PCNA-K107 mediated cell cycle arrest PMID: 23824283
  2. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination PMID: 27130983
  3. We show that Rad59-facilitated Y' translocations on X-only telomeres delay the onset of senescence while preparing ground for type I survivor formation. PMID: 25375789
  4. Findings suggest a Rad52/Rad59-dependent, recombination pathway that could ultimately lead to the removal of faulty flaps in conjunction with cohesion establishment factors. PMID: 24711454
  5. These results suggest that Rad59 influences the rescue of replication lesions by multiple recombination factors. PMID: 24125552
  6. Data show that RSC physically interacts with the recombination protein Rad59 and functions in homologous recombination. PMID: 21807899
  7. at least one role of Rad59 in translocation formation by single-strand annealing is supporting the machinery required for cleavage of non-homologous tails PMID: 20012294
  8. examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties. PMID: 16565518
  9. S. cerevisiae Rad59 protein and its eukaryotic counterparts function by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break. PMID: 17040915
  10. Data from this study show that defects in the Rad51-Sgs1-dependent and Sgs1-dependent lesion-bypass pathways activate Rad59-Rad1- and Rad59-dependent pathways, respectively, resulting in uSCR. PMID: 17399687
  11. RAD59 was found to play a particularly significant role in recombination between the short substrates that was partially independent of that of Rad52 DNA Repair and Recombination Protein PMID: 18373960

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Subcellular Location
Nucleus.
Protein Families
RAD52 family
Database Links

KEGG: sce:YDL059C

STRING: 4932.YDL059C

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