Recombinant Saccharomyces cerevisiae DNA repair protein RAD59 (RAD59)

Code CSB-EP619572SVG
Abbreviation Recombinant Saccharomyces cerevisiae RAD59 protein
MSDS
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
RAD59
Uniprot No.
Research Area
Others
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Source
E.coli
Expression Region
1-238aa
Target Protein Sequence
MTIQAKPSSSISYDSTTYGTAPGLDIKEFQIIEDWNGRPASAWSVQRIGLLQSKIERYTYNIYHNNKYGKHNLSKLIPGHALIQFANETFGYDGWRMDVIDVEARECQPFTAVNNGENTNTSEVKYTVVAEAQVKVTLKDGTNTQCGGLGRITLSSRGECYNRSKKEAVGDALKKALLSFEKIILDYETKITNNYYVDGLYGSKKIKNEANTNYNLLSATNSKPTFIKLEDAKGTHIK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
33.5
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing. Anneals complementary single-stranded DNA.
Gene References into Functions
  1. propose that Rad59 promotes fork progression when Okazaki fragment processing is compromised and counteracts PCNA-K107 mediated cell cycle arrest PMID: 23824283
  2. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination PMID: 27130983
  3. We show that Rad59-facilitated Y' translocations on X-only telomeres delay the onset of senescence while preparing ground for type I survivor formation. PMID: 25375789
  4. Findings suggest a Rad52/Rad59-dependent, recombination pathway that could ultimately lead to the removal of faulty flaps in conjunction with cohesion establishment factors. PMID: 24711454
  5. These results suggest that Rad59 influences the rescue of replication lesions by multiple recombination factors. PMID: 24125552
  6. Data show that RSC physically interacts with the recombination protein Rad59 and functions in homologous recombination. PMID: 21807899
  7. at least one role of Rad59 in translocation formation by single-strand annealing is supporting the machinery required for cleavage of non-homologous tails PMID: 20012294
  8. examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties. PMID: 16565518
  9. S. cerevisiae Rad59 protein and its eukaryotic counterparts function by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break. PMID: 17040915
  10. Data from this study show that defects in the Rad51-Sgs1-dependent and Sgs1-dependent lesion-bypass pathways activate Rad59-Rad1- and Rad59-dependent pathways, respectively, resulting in uSCR. PMID: 17399687
  11. RAD59 was found to play a particularly significant role in recombination between the short substrates that was partially independent of that of Rad52 DNA Repair and Recombination Protein PMID: 18373960

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Subcellular Location
Nucleus.
Protein Families
RAD52 family
Database Links

KEGG: sce:YDL059C

STRING: 4932.YDL059C

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