RABF2B Antibody

Datasheet

Code	CSB-PA871121XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RABF2B Polyclonal antibody

Uniprot No.

Q9SN68

Target Names

RABF2B

Alternative Names

RABF2B antibody; ARA-7 antibody; RAB5B antibody; At4g19640 antibody; F24J7.190 antibody; Ras-related protein RABF2b antibody; AtRABF2b antibody; Ras-related protein Ara-7 antibody; Ras-related protein Rab5B antibody; AtRab5B antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RABF2B protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Endosomal protein that may be involved in endocytosis. Involved in the trafficking of proteins from prevacuolar compartments (PVCs) to vacuoles. May activate the MON1-CCZ1 complex which acts as guanine nucleotide exchange factors (GEF) for Rab7 protein family, and serves as a link between Rab5 and Rab7 families in PVCs, and mediates PVC maturation. Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs).

Gene References into Functions

VHA-a3 targets to the tonoplast through a Rab5-mediated but Rab7-independent pathway, shedding new lights on the unconventional vacuolar trafficking route in plant cells.[Rab5] PMID: 28198146
Ara7 was localized to the the extrahaustorial membrane during powdery mildew infection. PMID: 27318282
A loss of FAB1 function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane, indicating that FAB1, or its product PtdIns(3,5)P2, mediates the maturation process of the late endosomes. PMID: 26353760
ARA7(Q69L) overexpression induces the formation of enlarged multivesicular bodies. PMID: 23682115
The crystal structure of ARA7-GDP-divalent calcium ion (Ca2+)-VPS9a reveals that the metal ion directly interacts with the aspartate finger of VPS9a. PMID: 23519409

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Subcellular Location

Early endosome membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Prevacuolar compartment membrane; Lipid-anchor. Endosome, multivesicular body membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

Expressed in roots and actively dividing cells.

Database Links

KEGG: ath:AT4G19640

STRING: 3702.AT4G19640.1

UniGene: At.2030

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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RABF2B Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background