Recombinant Arabidopsis thaliana Ras-related protein RABF2b (RABF2B)

Code CSB-YP871121DOA
MSDS
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Source Yeast
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Code CSB-EP871121DOA
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Source E.coli
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Code CSB-EP871121DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871121DOA
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Source Baculovirus
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Code CSB-MP871121DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RABF2B
Uniprot No.
Alternative Names
RABF2B; ARA-7; RAB5B; At4g19640; F24J7.190; Ras-related protein RABF2b; AtRABF2b; Ras-related protein Ara-7; Ras-related protein Rab5B; AtRab5B
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-200
Target Protein Sequence
MAAAGNKSIN AKLVLLGDVG AGKSSLVLRF VKDQFVEFQE STIGAAFFSQ TLAVNDATVK FEIWDTAGQE RYHSLAPMYY RGAAAAIIVF DVTNQASFER AKKWVQELQA QGNPNMVMAL AGNKSDLLDA RKVTAEDAQT YAQENGLFFM ETSAKTATNV KEIFYEIARR LPRVQPTENP TGMVLPDRAM DRAVSSSCCA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Endosomal protein that may be involved in endocytosis. Involved in the trafficking of proteins from prevacuolar compartments (PVCs) to vacuoles. May activate the MON1-CCZ1 complex which acts as guanine nucleotide exchange factors (GEF) for Rab7 protein family, and serves as a link between Rab5 and Rab7 families in PVCs, and mediates PVC maturation. Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs).
Gene References into Functions
  1. VHA-a3 targets to the tonoplast through a Rab5-mediated but Rab7-independent pathway, shedding new lights on the unconventional vacuolar trafficking route in plant cells.[Rab5] PMID: 28198146
  2. Ara7 was localized to the the extrahaustorial membrane during powdery mildew infection. PMID: 27318282
  3. A loss of FAB1 function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane, indicating that FAB1, or its product PtdIns(3,5)P2, mediates the maturation process of the late endosomes. PMID: 26353760
  4. ARA7(Q69L) overexpression induces the formation of enlarged multivesicular bodies. PMID: 23682115
  5. The crystal structure of ARA7-GDP-divalent calcium ion (Ca2+)-VPS9a reveals that the metal ion directly interacts with the aspartate finger of VPS9a. PMID: 23519409

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Subcellular Location
Early endosome membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Prevacuolar compartment membrane; Lipid-anchor. Endosome, multivesicular body membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm.
Protein Families
Small GTPase superfamily, Rab family
Tissue Specificity
Expressed in roots and actively dividing cells.
Database Links

KEGG: ath:AT4G19640

STRING: 3702.AT4G19640.1

UniGene: At.2030

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