ran-1 Antibody

Code CSB-PA517713XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans ran-1 Polyclonal antibody
Uniprot No.
Target Names
ran-1
Alternative Names
ran-1 antibody; K01G5.4 antibody; GTP-binding nuclear protein ran-1 antibody; Ras-related nuclear protein 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans ran-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ran GTPase system comprises ran-1, ran-2 and ran-3 and is essential in nucleocytoplasmic transport. Ran-1 is a GTP-binding protein that mediates the interaction between mitotic chromosomes and kinetochore microtubules. Plays a crucial role in nuclear envelope assembly at the end of each cell division. Required for the import of protein into the nucleus and also for RNA export. RCC1 (ran-3)/Ran (ran-1) complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA.
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Chromosome. Note=At interphase, ran-1 is localized to the nucleus and the nuclear envelope. At prometaphase, ran-1 is still localized to the nuclear envelope. When the nuclear envelope begins to disassemble at M phase (early metaphase), ran-1 is localized throughout the entire cell. At late metaphase, ran-1 localizes to the kinetochore regions, this persists until anaphase. At telophase, ran-1 is localized around the decondensing chromosomes.
Protein Families
Small GTPase superfamily, Ran family
Database Links

KEGG: cel:CELE_K01G5.4

STRING: 6239.K01G5.4.1

UniGene: Cel.17475

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