Recombinant GTP-binding nuclear protein ran-1 (ran-1)

Code CSB-YP517713CXY
MSDS
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Source Yeast
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Code CSB-EP517713CXY
MSDS
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Source E.coli
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Code CSB-EP517713CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517713CXY
MSDS
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Source Baculovirus
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Code CSB-MP517713CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ran-1
Uniprot No.
Alternative Names
ran-1; K01G5.4; GTP-binding nuclear protein ran-1; Ras-related nuclear protein 1
Species
Caenorhabditis elegans
Expression Region
1-215
Target Protein Sequence
MSGGDGIPTF KLVLVGDGGT GKTTFVKRHL TGEFEKKYVA TLGVEVHPLV FHTNRGQIRF NVWDTAGQEK FGGLRDGYYI QGQCAIIMFD VTARVTYKNV PNWHRDLARV CENIPIVLCG NKVDVKDRKV KAKTITFHRK KNLQYYDISA KSNYNFEKPF LWLARKLLGD PNLEFVAMPA LAPPEVQMDP AMIAEYEKDL DNAAKADLPD DDDDL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ran GTPase system comprises ran-1, ran-2 and ran-3 and is essential in nucleocytoplasmic transport. Ran-1 is a GTP-binding protein that mediates the interaction between mitotic chromosomes and kinetochore microtubules. Plays a crucial role in nuclear envelope assembly at the end of each cell division. Required for the import of protein into the nucleus and also for RNA export. RCC1 (ran-3)/Ran (ran-1) complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA.
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Chromosome. Note=At interphase, ran-1 is localized to the nucleus and the nuclear envelope. At prometaphase, ran-1 is still localized to the nuclear envelope. When the nuclear envelope begins to disassemble at M phase (early metaphase), ran-1 is localized throughout the entire cell. At late metaphase, ran-1 localizes to the kinetochore regions, this persists until anaphase. At telophase, ran-1 is localized around the decondensing chromosomes.
Protein Families
Small GTPase superfamily, Ran family
Database Links

KEGG: cel:CELE_K01G5.4

STRING: 6239.K01G5.4.1

UniGene: Cel.17475

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