Product Details

Full Product Name

Rabbit anti-Rattus norvegicus (Rat) Rab27a Polyclonal antibody

Uniprot No.

P23640

Target Names

Rab27a

Alternative Names

Rab27a antibody; Rab27 antibody; Ras-related protein Rab-27A antibody; Rab-27 antibody; EC 3.6.5.2 antibody; GTP-binding protein Ram antibody; Ram p25 antibody

Raised in

Rabbit

Species Reactivity

Rattus norvegicus (Rat)

Immunogen

Recombinant Rattus norvegicus (Rat) Rab27a protein

Immunogen Species

Rattus norvegicus (Rat)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Gene References into Functions

Data show that the intracellular M4M5 loop of human alpha1 subunit of Na+,K+-ATPase (ATP1a1) interacts directly with rat rab GTP-binding protein Rab27a. PMID: 25051489
These findings indicated that Madd functions as a guanine nucleotide exchange factor for Rab27 in parotid acinar cells and regulates isoproterenol-stimulated amylase release. PMID: 23702376
cyt b(558) depends on clathrin for internalization, and in activated macrophages NADPH oxidase occupies a Rab27A/B-regulated secretory compartment PMID: 22157766
Rab27A interacts with Rabphilin and Noc2 which are recruited to dense-core vesicles in rat cells PMID: 14722103
Results demonstrate that Rab27A plays a crucial role in antigen-induced histamine release from RBL-2H3 cells. PMID: 15465017
rab27a is a direct partner of munc13-4. The complex of the two proteins is an essential regulator of secretory granule fusion with the plasma membrane in hematopietic cells. Mutations in either of the two genes prevent formation of this compex. PMID: 15548590
Our results suggest that the Noc2/Rab27 complex is an important constituent of the early stages of IPR-stimulated amylase release. PMID: 17067543
Three Rab3/27 effectors, Granuphilin, Noc2, and Rabphilin, in PC12 cells using fluorescence recovery after photobleaching of EGFP-tagged proteins. PMID: 18573236

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Subcellular Location

Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.

Database Links

KEGG: rno:50645

STRING: 10116.ENSRNOP00000010012

UniGene: Rn.37360

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Rab27a Antibody

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