RAB27A

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Overlay Peak curve showing PC3 cells stained with CSB-RA285197A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA019177LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA019177LA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA019177LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)