Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RD21A Polyclonal antibody

Uniprot No.

P43297

Target Names

RD21A

Alternative Names

RD21A antibody; At1g47128 antibody; F2G19.31 antibody; Cysteine proteinase RD21A antibody; EC 3.4.22.- antibody; Protein RESPONSIVE TO DEHYDRATION 21 antibody; RD21 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RD21A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Cysteine protease that plays a role in immunity, senescence, and biotic and abiotic stresses. Involved in immunity against the necrotrophic fungal pathogen Botrytis cinerea. Involved in elicitor-stimulated programmed cell death (PCD). During infection by the necrotrophic fungal pathogen Botrytis cinerea, functions as PCD-promoting protease that is released from the ER body or vacuole to the cytoplasm. Accumulates in endoplasmic reticulum-derived bodies in epidermal cells and may participate in cell death in stressed or injured cells. Involved in water stress-induced cell death through its protease activity that is released to the cytoplasm after vacuolar collapse. Possesses protease activity in vitro and is involved in cell death in the transmitting tract and septum epidermis during flower development. Possesses peptide ligase activity. Can ligate peptides to unmodified N-termini of acceptor proteins. Probably ligates through a thioester intermediate.

Gene References into Functions

Serpin1 and WSCP differentially regulate the activity of RD21 during plant development in Arabidopsis thaliana. PMID: 28179567
WSCP physically interacts with RD21 and this interaction in turn inhibits the activity of RD21 as a pro-death protein. The regulatory circuit identified depends on the restricted expression of WSCP in the transmitting tract and the septum epidermis. PMID: 26160583
AtSerpin1 and RD21 can modulate necrotroph-induced cell death. PMID: 23398119
RD21 causes a dominant protease activity in Arabidopsis leaf extracts, responsible for SDS-induced proteome degradation PMID: 22396764
RD21 provides immunity to a necrotrophic pathogen. PMID: 22238602

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Subcellular Location

Vacuole. Golgi apparatus. Cytoplasm, Stress granule. Cytoplasm, P-body.

Protein Families

Peptidase C1 family

Database Links

KEGG: ath:AT1G47128

STRING: 3702.AT1G47128.1

UniGene: At.43549

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RD21A Antibody

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