Recombinant Arabidopsis thaliana Cysteine proteinase RD21a (RD21A)

Code CSB-YP331791DOA
MSDS
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Source Yeast
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Code CSB-EP331791DOA
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Source E.coli
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Code CSB-EP331791DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331791DOA
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Source Baculovirus
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Code CSB-MP331791DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RD21A
Uniprot No.
Alternative Names
RD21A; At1g47128; F2G19.31; Cysteine proteinase RD21A; EC 3.4.22.-; Protein RESPONSIVE TO DEHYDRATION 21; RD21
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
137-356
Target Protein Sequence
LPES IDWRKKGAVA EVKDQGGCGS CWAFSTIGAV EGINQIVTGD LITLSEQELV DCDTSYNEGC NGGLMDYAFE FIIKNGGIDT DKDYPYKGVD GTCDQIRKNA KVVTIDSYED VPTYSEESLK KAVAHQPISI AIEAGGRAFQ LYDSGIFDGS CGTQLDHGVV AVGYGTENGK DYWIVRNSWG KSWGESGYLR MARNIASSSG KCGIAIEPSY PIKNGE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cysteine protease that plays a role in immunity, senescence, and biotic and abiotic stresses. Involved in immunity against the necrotrophic fungal pathogen Botrytis cinerea. Involved in elicitor-stimulated programmed cell death (PCD). During infection by the necrotrophic fungal pathogen Botrytis cinerea, functions as PCD-promoting protease that is released from the ER body or vacuole to the cytoplasm. Accumulates in endoplasmic reticulum-derived bodies in epidermal cells and may participate in cell death in stressed or injured cells. Involved in water stress-induced cell death through its protease activity that is released to the cytoplasm after vacuolar collapse. Possesses protease activity in vitro and is involved in cell death in the transmitting tract and septum epidermis during flower development. Possesses peptide ligase activity. Can ligate peptides to unmodified N-termini of acceptor proteins. Probably ligates through a thioester intermediate.
Gene References into Functions
  1. Serpin1 and WSCP differentially regulate the activity of RD21 during plant development in Arabidopsis thaliana. PMID: 28179567
  2. WSCP physically interacts with RD21 and this interaction in turn inhibits the activity of RD21 as a pro-death protein. The regulatory circuit identified depends on the restricted expression of WSCP in the transmitting tract and the septum epidermis. PMID: 26160583
  3. AtSerpin1 and RD21 can modulate necrotroph-induced cell death. PMID: 23398119
  4. RD21 causes a dominant protease activity in Arabidopsis leaf extracts, responsible for SDS-induced proteome degradation PMID: 22396764
  5. RD21 provides immunity to a necrotrophic pathogen. PMID: 22238602

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Subcellular Location
Vacuole. Golgi apparatus. Cytoplasm, Stress granule. Cytoplasm, P-body.
Protein Families
Peptidase C1 family
Database Links

KEGG: ath:AT1G47128

STRING: 3702.AT1G47128.1

UniGene: At.43549

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