RFC1 Antibody

Code CSB-PA171509XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RFC1 Polyclonal antibody
Uniprot No.
Target Names
RFC1
Alternative Names
RFC1 antibody; At5g22010 antibody; T6G21 antibody; Replication factor C subunit 1 antibody; AtRFC1 antibody; Activator 1 large subunit antibody; Activator 1 subunit 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RFC1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a role as mediator of transcriptional gene silencing (TGS), DNA replication, DNA repair, hypersensitive response (HR) and telomere length regulation. Is required in meiosis for DNA double-strand break (DSB) repair during meiotic homologous recombination. May participate in the RAD51-mediated recombination intermediate repair process. Is important for lagging strand synthesis. Promotes meiotic recombination via a specific pathway for crossovers (COs) that involves the formation of double Holliday Junction (dHJ) intermediates.
Gene References into Functions
  1. required for double-strand break repair during meiotic homologous recombination PMID: 22974522
  2. RFC1 plays critical roles in abscisic acid-mediated homologous recombination in Arabidopsis. PMID: 24053948
  3. That RFC1 is found in divergent eukaryotes suggests a previously unrecognized and highly conserved role for DNA synthesis in discriminating between recombination pathways. PMID: 23144629
  4. results suggest that RFC1 helps mediate genomic stability and transcriptional gene silencing in Arabidopsis thaliana PMID: 20639449
  5. Dada suggest that AtRFC1 plays an important role in embryo development in Arabidopsis thaliana. PMID: 17556804

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Subcellular Location
Nucleus. Note=Localizes to the chromosomes during zygotene and pachytene.
Protein Families
Activator 1 large subunit family
Tissue Specificity
Expressed at high levels in flowers and siliques, and at lower levels in roots, stems and leaves.
Database Links

KEGG: ath:AT5G22010

STRING: 3702.AT5G22010.1

UniGene: At.45635

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