Y10A Antibody

^{Rare Species}

Datasheet

Code	CSB-PA327123XA01EDZ
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes:Y10A Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) Y10A Polyclonal antibody

Uniprot No.

P32277

Target Names

Y10A

Alternative Names

RNA ligase 2 (Rnl2) (EC 6.5.1.3) Y10A 24.1

Raised in

Rabbit

Species Reactivity

Enterobacteria phage T4 (Bacteriophage T4)

Immunogen

Recombinant Enterobacteria phage T4 (Bacteriophage T4) Y10A protein (1-249aa)

Immunogen Species

Enterobacteria phage T4 (Bacteriophage T4)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Repairs 3'-OH/5'-PO4 nicks in duplex RNA or RNA:DNA hybrid in which the broken 3'-OH strand is RNA. The nick ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nicked duplex nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated nicked intermediate. In step 3, the RNA ligase directs the attack of the nick 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3' - 5' phosphodiester and release of AMP.

Gene References into Functions

To unravel the biochemical and functional relationship between Rnl1, Rnl2, and Dnl, a systematic analysis of their substrate specificity was performed using recombinant proteins PMID: 16671895
Results report crystal structures for T4 RNA ligase 2 (Rnl2) at discrete steps along the RNA repair pathway. PMID: 17018278

Protein Families

RNA ligase 2 family

Database Links

KEGG: vg:1258563

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Y10A Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background