ROPGEF1 Antibody

Code CSB-PA850049XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ROPGEF1 Polyclonal antibody
Uniprot No.
Target Names
ROPGEF1
Alternative Names
ROPGEF1 antibody; At4g38430 antibody; F22I13.200 antibody; Rop guanine nucleotide exchange factor 1 antibody; AtRopGEF1 antibody; Kinase partner protein-like antibody; KPP-like antibody; Rho of plants guanine nucleotide exchange factor 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ROPGEF1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Acts downstream of PRK2 in the control of polarized pollen tube growth by activating ARAC11/ROP1. In association with ROPGEF4, acts as specific regulator of ARAC10/ROP11 function in ABA-mediated stomatal closure. May play a role in the Rac/Rop-signaling pathway that controls ROS-mediated root hair development.
Gene References into Functions
  1. critical for AUX1 localization and PIN differential accumulation; role in cell polarity and polar auxin transport whereby it impacts auxin-mediated plant growth and development PMID: 28698357
  2. PP2C protein phosphatases protect and stabilize RopGEF1 from abscisic acid -mediated degradation.RopGEF1 is epistatic to PP2C phosphatases in abscisic acid signal transduction pathway.RopGEF1 plays an important role in abscisic acid -mediated inhibition of lateral root growth. PMID: 27192441
Subcellular Location
Cytoplasm, cytosol. Cell membrane.
Tissue Specificity
Expressed in roots, cotyledons, leaves, stems, sepals, petals, anthers, pollen grains, stigmas and siliques.
Database Links

KEGG: ath:AT4G38430

STRING: 3702.AT4G38430.1

UniGene: At.2769

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