Recombinant Arabidopsis thaliana Rop guanine nucleotide exchange factor 1 (ROPGEF1), partial

Code CSB-YP850049DOA
MSDS
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Source Yeast
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Code CSB-EP850049DOA
MSDS
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Source E.coli
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Code CSB-EP850049DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP850049DOA
MSDS
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Source Baculovirus
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Code CSB-MP850049DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ROPGEF1
Uniprot No.
Alternative Names
ROPGEF1; At4g38430; F22I13.200; Rop guanine nucleotide exchange factor 1; AtRopGEF1; Kinase partner protein-like; KPP-like; Rho of plants guanine nucleotide exchange factor 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Acts downstream of PRK2 in the control of polarized pollen tube growth by activating ARAC11/ROP1. In association with ROPGEF4, acts as specific regulator of ARAC10/ROP11 function in ABA-mediated stomatal closure. May play a role in the Rac/Rop-signaling pathway that controls ROS-mediated root hair development.
Gene References into Functions
  1. critical for AUX1 localization and PIN differential accumulation; role in cell polarity and polar auxin transport whereby it impacts auxin-mediated plant growth and development PMID: 28698357
  2. PP2C protein phosphatases protect and stabilize RopGEF1 from abscisic acid -mediated degradation.RopGEF1 is epistatic to PP2C phosphatases in abscisic acid signal transduction pathway.RopGEF1 plays an important role in abscisic acid -mediated inhibition of lateral root growth. PMID: 27192441
Subcellular Location
Cytoplasm, cytosol. Cell membrane.
Tissue Specificity
Expressed in roots, cotyledons, leaves, stems, sepals, petals, anthers, pollen grains, stigmas and siliques.
Database Links

KEGG: ath:AT4G38430

STRING: 3702.AT4G38430.1

UniGene: At.2769

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