Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans rpm-1 Polyclonal antibody

Uniprot No.

Q17551

Target Names

rpm-1

Alternative Names

rpm-1 antibody; syd-3 antibody; C01B7.6 antibody; E3 ubiquitin-protein ligase rpm-1 antibody; EC 2.3.2.- antibody; Pam/highwire/rpm-1 protein antibody; Regulator of presynaptic morphology protein 1 antibody; Synapse defective protein 3 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans rpm-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues. Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. Mediates ubiquitination and subsequent proteasomal degradation of target proteins, including dlk-1. Negatively regulates a p38 MAP kinase pathway composed of dlk-1, mkk-4, and pmk-3 that functions presynaptically to regulate synaptic architecture. Has a role during synaptogenesis; regulates neuronal morphology, specifically presynaptic differentiation. May regulate the spatial arrangement of presynaptic terminals or restrict their development. By targeting both dlk-1 and mlk-1 for degradation, may negatively regulate mlk-1/mek-1/kgb-1 and dlk-1/mkk-4/pmk-3 pathways which are involved in axon regeneration after injury. Regulates axon termination in PLM and ALM neurons. May play a role in the formation of muscle connections between the body wall and the motor axons in the dorsal and ventral cord.

Gene References into Functions

The findings provide insight into how growth cone collapse is regulated during axon termination in vivo, and suggest that RPM-1 signaling destabilizes microtubules to facilitate growth cone collapse and axon termination. PMID: 29084805
results indicate that RPM-1 uses different mechanisms to recruit FSN-1 and RAE-1 into independent signaling complexes in neurons PMID: 27979965
The findings show that axon termination defects are a core phenotype caused by loss of function in rpm-1. PMID: 24885325
RPM-1 negatively regulates a p38 MAP kinase pathway composed of the dual leucine zipper-bearing MAPKKK DLK-1, the MAPKK MKK-4, and the p38 MAP kinase PMK-3. PMID: 15707898
Our data suggest that RPM-1 positively regulates a Rab GTPase pathway to promote vesicular trafficking via late endosomes. PMID: 17698012
findings show that in mature neurons, RPM-1 resides in a distinct region that is close to, but does not overlap with, the synaptic exo- and endocytosis domains PMID: 18224716
RPM-1 regulates axon outgrowth affecting axon guidance and termination by controlling the trafficking of the UNC-5 and SAX-3 receptors to cell membranes. PMID: 18385318

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Subcellular Location

Cell projection, axon.

Protein Families

Highwire family

Tissue Specificity

Expression is seen in the pharynx, coelomocytes and distal tip cell. Most abundant expression is in axons of the nerve ring neuropil. Expression is not seen in body wall muscle, hypodermis or intestine.

Database Links

KEGG: cel:CELE_C01B7.6

STRING: 6239.C01B7.6

UniGene: Cel.2746

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rpm-1 Antibody

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